huprot protein array service  (Cambridge Protein Arrays)

Journal: Microbiology Spectrum

Article Title: Identification of a mimotope of a complex gp41 human immunodeficiency virus epitope related to a non-structural protein of Hepacivirus previously implicated in Kawasaki disease

doi: 10.1128/spectrum.01911-24

Figure Lengend Snippet: Binding to yeast displayed human proteins. (A) Results of the full library interrogation for 23059 Saccharomyces cerevisiae expressed and purified human proteins (HuProt library, CDI labs) are displayed, for 76Canc (black) and 6F5 (gray). (B) ELISA confirms low-level binding with representative and normalized data of three experiments showing means and standard errors. (C) Western blot (WB) is aligned adjacent to the Coomassie blue stain. (D) Native protein was blotted in a dot blot format with areas of inoculation at the pen marks in the center of the circle. Representative blots are shown of experiments repeated at least twice.

Article Snippet: Serum samples were provided to CDI laboratories to screen on the HuProt array.

Techniques: Binding Assay, Purification, Enzyme-linked Immunosorbent Assay, Western Blot, Staining, Dot Blot

Journal: Cell Death & Disease

Article Title: BACH1-induced ferroptosis drives lymphatic metastasis by repressing the biosynthesis of monounsaturated fatty acids

doi: 10.1038/s41419-023-05571-z

Figure Lengend Snippet: A Expression patterns of ferroptosis-related genes in tumors with preferential lymphatic metastasis or nonlymphatic/hematogenous metastasis. The red color indicates overexpression in respective tumors, and the sky-blue color indicates downregulation in respective tumors, whereas grey indicates no difference between tumor and nontumor tissues (FDR-adjusted Wilcox test). The blocks beneath the TCGA RNA sequencing datasets indicate the classifications of metastatic types. Some key ferroptosis regulatory genes are labeled; among them, BACH1 was overexpressed in tumors with preferential lymphatic metastasis (PLM). SKCM, skin cutaneous melanoma (metastatic lesions vs. primary tumors); ESCA, esophageal carcinoma; HNSC, head and neck cancer; STAD, stomach adenocarcinoma; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; THCA, thyroid carcinoma; PRAD, prostate adenocarcinoma; KICH, kidney chromophobe; LUAD, lung adenocarcinoma; BRCA, breast invasive carcinoma; BLCA, bladder urothelial carcinoma; COAD, colon adenocarcinoma; READ, rectum adenocarcinoma; LUSC, lung squamous cell carcinoma; UCEC, uterine corpus endometrial carcinoma; LIHC, liver hepatocellular carcinoma; PLM, tumors with preferential lymphatic metastasis; non-PLM, tumors with preferential nonlymphatic metastasis. B Whole-proteome screening for serum autoantibodies (AAbs) associated with the tumor and lymph node metastasis (LNM) in esophageal squamous cell carcinoma (ESCC). We employed a discovery strategy to identify serum AAb candidate biomarkers for early-stage ESCC using a proteome array-based approach. The screened serum samples were collected from healthy controls (HC), T1-stage ESCC patients without lymph node metastasis (w/o LNM, T1N0M0) and T1-stage ESCC patients with lymph node metastasis (w/ LNM, T1N1M0). These sera were loaded onto a human proteome microarray of 19,394 recombinant proteins to acquire an AAb repertoire in response to ESCC and LNM. ORF, open reading frame. C Overlapping genes among the three groups (ESCC vs. HC AAbs, LNM-associated AAbs and ferroptosis-related genes). BACH1 was the only shared gene among the three groups. D Heatmap of overlapping genes in in tumors with preferential lymphatic metastasis or nonlymphatic/hematogenous metastasis. E The discriminatory ability (sensitivity + specificity) and fold changes of the upregulated AAbs on the HuProt arrays in the IgG channel. Each dot represents an AAb. Anti-BACH1 AAbs completely distinguished patients with LNM from those without LNM. F Typical anti-BACH1 IgG AAb scanning image and fluorescence intensity quantification of the microarray. G BACH1 showed a strong anti-human IgG signal in patients with ESCC w/ LNM but a weak signal in healthy controls (HC) and patients with ESCC w/o LNM.

Article Snippet: HuProt proteome arrays (CDI Laboratories, Baltimore, MD) were employed to screen serum autoantibodies of ESCC patients.

Techniques: Expressing, Over Expression, RNA Sequencing, Labeling, Microarray, Recombinant, Fluorescence